The transplantation of autologous BM-MSCs holds great potential for treating end-stage liver diseases. The aim of this study\nwas to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs)\nfor suppression of dimethylnitrosamine-injured liver damage in rat model. Synchrotron radiation Fourier-transform infrared\n(SR-FTIR) microspectroscopy was applied to investigate changes in the macromolecular composition. Transplantation of\nrBM-MSC-DSCs into liver-injured rats restored their serum albumin level and significantly suppressed transaminase activity\nas well as the morphological manifestations of liver disease. The regenerative effects of rBM-MSC-DSCs were corroborated\nunequivocally by the phenotypic difference analysis between liver tissues revealed by infrared spectroscopy. Spectroscopic\nchanges in the spectral region from 1190ââ?¬â??970 cmâË?â??1 (bands with absorbance maxima at 1150 cmâË?â??1, 1081 cmâË?â??1, and\n1026 cmâË?â??1) indicated decreased levels of carbohydrates, in rBM-MSC-DSC-transplanted livers, compared with untreated and\nrBM-MSCââ?¬â??transplanted animals. Principal component analysis (PCA) of spectra acquired from liver tissue could readily\ndiscriminate rBM-MSC-DSC-transplanted animals from the untreated and rBM-MSC-transplanted animals. We conclude\nthat the transplantation of rBM-MSC-DSCs effectively treats liver disease in rats and SR-FTIR microspectroscopy provides\nimportant insights into the fundamental biochemical alterations induced by the stem-derived cell transplantation, including\nan objective ââ?¬Å?signatureââ?¬Â of the regenerative effects of stem cell therapy upon liver injury.
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